The procedure to obtain PRP begins by doing a blood sample on an anticoagulated tube . The tubes are centrifuged for about 5 to 10 minutes at 600– 1200 G. (Speed and time depend on the kit protocol.) According to their density, the red cells go down in the tube . On the upper part, we have the plasma and the platelets. We usually talk about PRP when we have a concentration three to seven times normal standards.
The contraindications are cervicofacial cancer history, hematologic diseases, pregnancy, active local infection, and anticoagulant treatment. We have to pay attention to history of allergies because activated platelets also release histamine.
The prevalence of progressive AGA approaches 50% of Caucasian men and women beyond the age of 40; whereas in Asian, Native American, and African American men the prevalence is lower and AGA is less severe.
METHOD OF USE IN HAIR RESTORATION SURGERY
Phase I: Harvesting
PRP in donor site strip harvesting and follicular unit transplantation FUT
After the subcuticular layer is closed with 3 Monocryl, PRP gel is injected into the wound edges from end to end. The second layer is approximated with a running 3 Prolene suture, and PRP is then injected back into the wound.
PRP in follicular unit extraction follicular unit transplantation FUE
Since individual follicular units are extracted in the FUE procedure, PRP is injected into the donor site before extraction to promote hemostasis.
Grafts cultured in PRP
After the follicular units are dissected, they are cultured and bathed in PRP prior to implantation so the growth factors can attach to the stem cells in the bulge region of the dissected follicular units, PRP improved follicle vascularization ,promotes hair growth and increases follicle and hair size.
Injecting PRP into the recipient area have advantages for the nontransplanted hairs.PRP enhance the procedure by decreasing scaring and promote preservation of the donor site, increase the yield of grafts and promote hair follicle reconstitution.